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from basic science:
to the translational research:
Etienne Morel

Focus

LabCloud

The main focus of our research team is to understand how mammalian cells mobilize organelles, endomembrane-based signaling platforms and autophagic machinery to adapt to stress situations and external stimuli.

More information / follow us on:
https://www.researchgate.net/lab/Etienne-Morel-Lab-autophagy-pathway-and-intracellular-membrane-dynamics-Etienne-Morel

https://twitter.com/MorelLab4

Introduction

Mammalian intracellular compartments are highly regulated and renewed to ensure the maintaining of proper cell physiology. In this dynamic context, the intracellular degradative autophagy pathway probably takes advantages of – and initiates dialogs with - pre-existing signaling platforms, organelles and endomembranes. Although there is low-level basal activity, autophagy is mostly associated with stress responses and is considered as an acute protective system that ensures cell homeostasis and contributes directly to cell metabolism and energy regulation. Accordingly, autophagy participates in development, immunity and protects against cell modifications related to ageing. Autophagy involves the formation, and subsequent trafficking, of autophagosomes, which arise from the closure of transient cup-shaped double-membrane structure termed phagophore that capture cytoplasmic cargos for a final delivery to lysosomes. Studying the crosstalk of autophagic machinery with other cellular organelles and structures, such as mitochondria, endosomes and primary cilia, will shed light on the importance of endomembranes and signaling platforms cooperation in stress sensing and homeostasis regulation in a wide variety of mammalian cells.

Research objectives

Our project aims at investigating the cellular and molecular mechanisms that regulate autophagic pathway during stress response in cooperation with other endomembranes, in physiological and pathophysiological situations.

3 interconnected axes are currently investigated in my laboratory:

  1. Space and time orchestration of autophagy associated membrane dynamics processes: roles of phosphoinositides and lipid-kinases, scaffolding proteins, cytoskeleton and membrane contact sites
  2. Cannonical and non-cannonical functions of autophagic machinery during the crosstalk with the primary cilium
  3. Non-cell autonomous functions of mechanical stress induced autophagy in kidney epithelial cells differentiation and metabolism, in normal and pathological situations

Keywords: autophagic membranes and machinery, chemical, nutritional, infectious and mechanical stresses, primary cilium, endosomes, mitochondria, metabolism, membrane dynamics and contact-sites.

5 main publications

  • The autophagy protein ATG16L1 cooperates with IFT20 and INPP5E to regulate the turnover of phosphoinositides at the primary cilium
    Boukhalfa A, Roccio F, Dupont N, Codogno P and Morel E
    Cell Reports 2021 Apr
  • Chemical targeting of NEET proteins reveals their function in mitochondrial morphodynamics
    Molino D*, Pila-Castellanos I*, Marjault HB, Amoedo ND, Kopp K, Rochin L, Karmi O, Sohn YS, Lines L, Hamaï A, Joly S, Radreau P, Vonderscher J, Codogno P, Giordano F, Machin P, Rossignol R, Meldrum E, Arnoult D, Ruggieri A, Nechushtai R, De Chassey B and Morel E
    EMBO Reports 2020 Nov
  • The primary cilium and lipophagy translate mechanical forces to direct metabolic adaptation of kidney epithelial cells
    Miceli C*, Penalva-Mousset L*, Roccio F*, Burtin M, Leroy C, Nemazanyy I, Friedlander G, Morel E, Terzi F, Codogno P and Dupont N
    Nature Cell Biology, 2020 Aug
  • PI3KC2α-dependent and VPS34-independent generation of PI3P controls primary cilium-mediated autophagy in response to shear stress
    Boukhalfa A*, Nascimbeni AC*, Ramel D, Dupont N, Hirsch E, Gayral S, Laffargue M#, Codogno P and Morel E
    Nature Communications, 2020 Jan
  • ER-plasma membrane contact sites contribute to autophagosome biogenesis by regulation of local PI3P synthesis
    Nascimbeni AC, Giordano F, Dupont N, Grasso D, Vaccaro MI, Codogno P and Morel E.
    EMBO Journal, 2017 May
30 last publications

2022

  • Membrane protective role of autophagic machinery during infection of epithelial cells by Candida albicans
    Lapaquette P, Ducreux A, Basmaciyan L, Paradis T, Bon F, Bataille A, Winckler P, Hube B, d'Enfert C, Esclatine A, Dubus E, Bringer MA, Morel E and Dalle F
    Gut Microbes 2022 Jan
  • 2021

  • Role of mitochondrial morphodynamics and contact sites in the antiviral response
    De Chassey B and Morel E
    Médecine/Sciences 2021 Dec
  • Primary cilium-dependent autophagy in the response to shear stress
    Morel E, Dupont N and Codogno P
    Biochemistry Society Transactions 2021 Dec
  • ATG9A protects plasma membrane from programmed and incidental permeabilization
    Claude-Taupin A, Jia J, Bhujabal Z, Garfa-Traoré M, Kumar S, Peixoto Duarte da Silva G, Javed R, Gu Y, Allers L, Peters R, Wang F, Jesus da Costa L, Pallikkuth S, Lidke KQ, Mauthe M, Verlhac P, Uchiyama Y, Salemi M, Phinney B, Tooze S.A, Mari M.C, Johansen T, Reggiori F, and Deretic V
    Nature Cell Biology 2021 June
  • When the autophagy protein ATG16L1 met the ciliary protein IFT20
    Boukhalfa A, Roccio F, Dupont N, Codogno P and Morel E
    Autophagy 2021 May
  • The autophagy protein ATG16L1 cooperates with IFT20 and INPP5E to regulate the turnover of phosphoinositides at the primary cilium
    Boukhalfa A, Roccio F, Dupont N, Codogno P# and Morel E#
    Cell Reports 2021 Apr
  • ATG4D is the main ATG8 delipidating enzyme in mammalian cells and protects against cerebellar neurodegeneration
    Tamargo-Gómez I, Martínez-García G, Suárez M, Rey V, Fueyo A, Codina-Martínez H, Tomás-Zapico C, Bretones G, M. Caravia X, Morel E, Dupont N, Cabo R, Souquere S, Pierron G, Codogno P, López-Otín C, Fernández A, and Mariño G
    Cell Death & Differentiation 2021 Apr
  • The ménage à trois of autophagy, lipid droplets and liver disease
    Filali-Mouncef Y, Hunter C, Roccio F, Zagkou S, Dupont N, Primard C, Proikas-Cezanne T, Reggiori F
    Autophagy 2021 Apr
  • Monitoring lipophagy in kidney epithelial cells in response to shear stress
    Roccio F, Claude-Taupin A, Botti J, Morel E, Codogno P and Dupont N
    Methods in Cell Biology 2021 March
  • Mitochondrial morphodynamics alteration induced by influenza virus infection as a new antiviral strategy
    Pila-Castellanos I*, Molino D*, McKellar J, Lines L, Da Graca J, Tauziet M, Chanteloup L,  IMikaelian, Meyniel-Schicklin L, Codogno P, Vonderscher J, Delevoye C, Moncorgé O, Meldrum E, Goujon C, Morel E# and De Chassey B#
    PLoS Pathogens 2021 Feb
  • Phosphoinositides: functions in autophagy-related stress responses
    Claude-Taupin A and Morel E
    BBA Molecular and Cellular Biology of Lipids 2021 Feb
  • The lipid kinase hidden behind the PI3P: a specific dialog between the primary cilium and autophagic machinery
    Codogno P, Dupont N and Morel E
    Medecine/Sciences 2021 Jan
  • 2020

  • Fluid flow-induced shear stress controls the metabolism of proximal tubule kidney epithelial cells through primary cilium dependent lipophagy and mitochondrial biogenesis
    Miceli C*, Roccio F*, Penalva-Mousset L*, Morel E, Codogno P# and Dupont N#
    Autophagy 2020 Dec
  • Chemical targeting of NEET proteins reveals their function in mitochondrial morphodynamics
    Molino D*, Pila-Castellanos I*, Marjault HB, Amoedo ND, Kopp K, Rochin L, Karmi O, Sohn YS, Lines L, Hamaï A, Joly S, Radreau P, Vonderscher J, Codogno P, Giordano F, Machin P, Rossignol R, Meldrum E, Arnoult D, Ruggieri A, Nechushtai R, De Chassey B# and Morel E#
    EMBO Reports 2020 Nov
  • The primary cilium and lipophagy translate mechanical forces to direct metabolic adaptation ; of kidney epithelial cells
    Miceli C*, Penalva-Mousset L*, Roccio F*, Burtin M, Leroy C, Nemazanyy I, Friedlander G, Morel E, Terzi F, Codogno P# and Dupont N#
    Nature Cell Biology 2020 Aug
  • Ultrastructural and dynamic studies of the endosomal compartment in Down syndrome
    Botté A, Lainé J, Xicota L, Heiligenstein X, Fontaine G, Kasri A, Rivals I, Goh, P, Faklaris O, Cossec JC, Morel E, Rebillat AS, Nizetic D, Raposo G and Potier MC
    Acta Neuropathologica Communications 2020 July
  • Primary cilium-dependent autophagy drafts PIK3C2A to generate PI3P in response to shear stress
    Boukhalfa A*, Nascimbeni AC*, Dupont N, Codogno P and Morel E
    Autophagy 2020 June
  • ER membrane and contact sites dynamics in autophagy regulation and stress response
    Morel E
    Frontiers in Cell and Developmental Biology 2020 May
  • PI3KC2α-dependent and VPS34-independent generation of PI3P controls primary cilium-mediated autophagy in response to shear stress
    Boukhalfa A*, Nascimbeni AC*, Ramel D, Dupont N, Hirsch E, Gayral S, Laffargue M, Codogno P# and Morel E#
    Nature Communications 2020 Jan
  • 2019

  • Interplay between primary cilia, ubiquitin-proteasome system and autophagy
    Boukhalfa A*, Miceli C*, Ávalos Y, Morel E and Dupont N
    Biochimie 2019 July
  • The primary cilium protein folliculin is part of the autophagy signalling pathway to regulate epithelial cell size in response to fluid flow
    Zemirli N*, Boukhalfa A*, Dupont N, Botti J, Codogno P and Morel E
    Cell Stress 2019 Feb
  • Monitoring of Autophagy and Cell Volume Regulation in Kidney Epithelial Cells in Response to Fluid Shear Stress
    Lazari MM, Orhon I, Codogno P, Dupont N.
    Methods Mol Biol. 2019
  • Autophagy is required for memory formation and reverses age-related memory decline
    Glatigny M*, Moriceau S*, Ramos-Brossier M*, Nascimbeni AC, Shanley MR, Rivagorda M, Boudarene N, Rousseaud A, Settembre C, Kuperwasser N, Friedlander G, Friedman AK, Morel E, Codogno P and Oury F.
    Current Biology 2019 Jan
  • 2018

  • A novel regulator of autophagosome biogenesis and lipid droplet dynamics
    Morel E and Codogno P.
    EMBO Reports 2018 Sept
  • FOXO3a provides a quickstep from autophagy inhibition to apoptosis in cancer therapy
    Codogno P and Morel E.
    Developmental Cell 2018 Mar
  • Mitochondrial dynamics in basal and stressful conditions
    Zemirli N, Morel E and Molino D.
    International Journal of Molecular Science 2018 Feb
  • Cholesterol trafficking and raft-like membrane domain composition mediate scavenger receptor class B type 1-dependent lipid sensing in intestinal epithelial cells
    Morel E, Ghezzal S, Lucchi G, Truntzer C, Pais de Barros JP, Simon-Plas F, Demignot S, Mineo C, Shaul PW, Leturque A, Rousset M and Carriere V.
    BBA Molecular And Cellular Biology of Lipids 2018 Feb
  • 2017

  • ER-driven membrane contact sites: evolutionary conserved machineries for stress response and autophagy regulation?
    Molino D, Nascimbeni AC, Giordano F, Codogno P and Morel E.
    Communicative & Integrative Biology 2017 Dec
  • To be or not be cell-autonomous? Autophagy says both
    Fenouille N, Nascimbeni AC, Botti-Millet J, Dupont N, Morel E and Codogno P
    Essays in Biochemistry 2017 Dec
  • Autophagy is required for endothelial cell alignment and atheroprotection under physiological blood flow.
    Vion AC, Kheloufi M, Hammoutene A, Poisson J, Lasselin J, Devue C, Pic I, Dupont N, Busse J, Stark K, Lafaurie-Janvore J, Barakat AI, Loyer X, Souyri M, Viollet B, Julia P, Tedgui A, Codogno P, Boulanger CM, Rautou PE.
    Proc Natl Acad Sci U S A. 2017 Oct
  • Local detection of PI3P at autophagosome biogenesis membrane platforms
    Nascimbeni AC, Codogno P and Morel E.
    Autophagy 2017 Sept
  • Autophagosomal membranes assemble at ER-plasma membrane contact sites
    Nascimbeni AC, Codogno P and Morel E.
    Molecular and Cellular Oncology 2017 July
  • Cellular and molecular mechanism for secretory autophagy.
    Kimura T, Jia J, Claude-Taupin A, Kumar S, Choi SW, Gu Y, Mudd M, Dupont N, Jiang S, Peters R, Farzam F, Jain A, Lidke KA, Adams CM, Johansen T, Deretic V.
    Autophagy. 2017 Jun
  • ER-plasma membrane contact sites contribute to autophagosome biogenesis ; by regulation of local PI3P synthesis
    Nascimbeni AC, Giordano F, Dupont N, Grasso D, Vaccaro MI, Codogno P and Morel E.
    EMBO Journal 2017 May
  • PI3P in the regulation of autophagy membrane dynamics
    Nascimbeni AC, Codogno P and Morel E.
    FEBS Journal 2017 May
  • The biogenesis of autophagosome: a new challenge for the cell biologist
    Morel E.
    Médecine/Sciences 2017 Mar
  • The journey of the autophagosome through mammalian cell organelles and membranes
    Molino D, Zemirli N, Codogno P and Morel E.
    Journal of Molecular Biology 2017 Feb
  • Autophagy: a Druggable Process
    Morel E, Mehrpour M, Botti J, Dupont N, Hamaï A, Nascimbeni AC and Codogno P.
    Annual Reviews in Pharmacology and Toxicology 2017 Jan
  • Etienne Morel is an INSERM research director (DR2) and group leader in the cell biology department of INEM. He obtained his PhD degree in Physiology and Cell Biology from Pierre et Marie Curie (Sorbonne) University in 2003 after 3 years as a graduate student in the lab of Jean Chambaz and Monique Rousset where he worked on lipoproteins trafficking and prion infection in epithelial cells. He then specialized on basic aspects of cellular biology by studying endosomal membrane dynamics in the lab of Jean Gruenberg in Geneva (Postdoc #1, 2004-2009, Dept. of Biochemistry of the University of Geneva) as well as in the biology of endosomal phosphoinositides in Alzheimer’s disease in the labs of Gilbert di Paolo and Scott Small in New York (Postdoc #2, 2009-2010, Cell Biology & Pathology Dept. of Columbia University). After having a permanent position at INSERM, he came back to France, at the Centre de Recherche des Cordeliers in Paris where he studied the role of autophagosome in neutral lipids behavior in enterocytes from 2010 to 2013. In 2014 he joined the research group of Patrice Codogno at the cell biology dept. of Institut Necker Enfants Malades to develop projects on basic autophagy in the context of stress sensing and he became group-leader of the “autophagy pathway and intracellular compartments dynamics” lab in 2019. The main goal of his research team is to understand how mammalian cells organelles and intracellular components cooperate with autophagic machinery to respond to stress stimuli. Recent data obtained in the lab identified several signaling platforms and organelles as key regulators of stress sensing and autophagic processes, such as the primary cilium, the mitochondria, the endosomal network or the membrane contact sites.

    https://sbcf.fr/member/etienne-morel/

    Permanent researcher
    Nicolas Dupont
    -
    +33 (0)1 40 61 54 02
    Etienne Morel
    Researcher
    +33 (0)1 40 61 54 00
    Post-doctoral degree
    Aurore Claude-Taupin
    Post-Doctoral Researcher
    +33 (0)1 40 61 54 00
    PhD
    Juliane Da Graca
    PhD Student
    +33 (0)1 44 61 54 04
    Student
    Carla Alemany
    Graduate student
    Sarah Dubois
    Graduate student
    Address

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    Support(s)
    HRH Princess Caroline of Hanover, who through the Princess Grace Foundation, already supports medical research and anything that helps to relieve the sick children in France and around the world, has agreed to commit to our side so that our Center of Molecular medicine continues to meet the current challenges and fight diseases, and in particular the ones affecting children.

    INEM - Organigramme