Bienvenu(e) sur le site de l'Institut Necker Enfants Malades
Offres d'emploi
Join our team: Postdoctoral position in Immunology - Mahévas Lab
Jeudi 4 janvier 2024
Scientific goal
Inflammatory bowel diseases (IBD), including Crohn’s disease (CD) and Ulcerative Colitis, are immune-related disorders characterized by inappropriate gut inflammation. Their incidence has increased dramatically in Western countries in recent decades. Despite the development of new treatments, IBD are insufficiently controlled in a large proportion of patients, inducing an alteration in the quality of life and a socioeconomic burden. A better understanding of the pathogenesis and identification of new therapeutic targets are therefore urgently needed.
Pathophysiology of CD is complex and not fully understood. While CD can affect the entire gastrointestinal tract, it primarily affects the terminal ileum. The underlying mechanistic reasons of this preferential location is unknown.
Patients with isolated ileal CD have higher prevalence of certain circulating IgG toward elements of the microbiota pointing toward a dysregulated humoral immune response. These antibodies are associated with the ileal location of CD as well as with a complicated phenotype (intestinal stenosis and fistulae). Cellular determinant of these humoral response in ileal CD are yet to be unraveled. Techniques to study B cells at the single cell levels have tremendously evolved these recent years and our laboratory has developed an expertise in most of these techniques.
The objectives of our study are to comprehensively characterize the humoral B cell response specifically in patients with isolated and/or complicated ileal CD.
Partners involved
Institut Necker Enfants Malades
INSERM
Université Paris Cité
Assistance Publique - Hôpitaux de Paris
Institut Mondor de Recherche Biomédicale
The post-doctoral scientist will work in the Mahevas’s Lab located at Institut Necker Enfants Malades (INEM) Research Center in the heart of Paris and at Institut Mondor de Recherche Biomédicale, in close collaboration with the clinical site (Hôpital Henri Mondor, Créteil).
Strategy
The study will be primarily performed from human sample, collected from ileal, colonic biopsies, and blood. Advanced B-cell focused single-cell techniques (including BCRseq, single-cell seq, FACS and B cell culture) mastered in our lab will be used.
Role Description
This is a full-time on-site role for a Postdoctoral Researcher in Immunology located at the INEM - Institut Necker Enfants Malades In Paris (Team Mahevas) and at Institut Mondor de Recherche Biomédicale (Team France Pirenne), in close collaboration with the clinical site (Hôpital Henri Mondor).
The Postdoctoral Researcher will be responsible for conducting independent research in immunology and applying advanced techniques to analyze complex data. They will be expected to collaborate with other researchers, PI of the project (Mathieu Uzzan) head of the lab (Matthieu Mahevas), senior scientists (Pascal Chappert, Jean Claude Weil), other postdocs, PhD students, engineers and technicians. They will also interact with other labs in France and abroad and with several platforms.
Selected candidate will attend seminars and meetings, and present their findings. Additionally, they will be responsible for writing scientific papers and grant proposals.
The position is vacant from March 2024 on a full-time basis and is integrated in an ambitious ANR-funded project (PI: Mathieu Uzzan)
Qualifications
  • Recent Ph.D. in Immunology or a related field
  • Strong knowledge in immunology
  • Experience in molecular biology, biochemistry, and cellular immunology techniques
  • Demonstrated expertise in designing and executing experiments, including flow cytometry and microscopy
  • Experience in data analysis
  • Excellent written and oral communication skills
  • Ability to work independently and collaboratively in a team environment
  • Strong organizational and time-management skills
  • Experience with manuscript and grant writing
Qualified candidates should e-mail before December 30th 2023 a cover letter, curriculum vitae, and list of two references to Dr Mathieu Uzzan at mathieuuzzan@gmail.com.
Join our team: Two-year postdoctoral position in autophagy, cell biology and renal physiology
Jeudi 4 janvier 2024
Institut Necker Enfants Malades (INEM) is a biomedical research center located on the Hôpital Necker-Enfants Malades campus. It benefits from numerous state-of-the-art core facilities. The Campus has a long-standing reputation for scientific excellence (8 principal investigators (PIs) at INEM received ERC awards) and key pioneering medical contributions (e.g. transplantation, biotherapy, gene therapy). It provides a vibrant environment for basic research and translational innovation.
The team Mechanisms and therapeutic strategies of chronic kidney disease is currently interested in the mechanisms by which autophagy integrates fluid shear stress in kidney epithelial cells (for example of our recent publication on the subject see: Orhon et al 2016 Nat Cell Biol 18, 657-67; Miceli C et al, Nat Cell Biol., 2020, 22:1091-1102, Claude-Taupin A, et al. Nat Commun. 2023, 2023 Dec 5;14(1):8056).
To carry out this project, biochemical, cellular and microfluidic approaches will be implemented in the laboratory as well as in vivo animal (mouse and zebrafish) models and human tissue samples in collaboration with the Necker Hospital.
In this context, the team is seeking a talented post-doctoral scientist to work on the role of autophagy in kidney epithelial cell homeostasis.
Your profile
We are seeking a highly motivated and creative candidates (m/f) with a strong drive for independent research.
Candidates with a Ph.D in molecular and cellular biology or in physiology obtained during the past four years and with experience in the fields of autophagy, cell signaling, kidney physiology, zebrafish model (not mandatory) are encouraged to apply.
The two-year position is open. Salary will be according to experience following INSERM regulation.
How to apply
Please send a concise cover letter with statement of research interest and summary of previous research activity, a detailed curriculum vitae and two reference letters to:
Nicolas Dupont - Team Mechanisms and therapeutic strategies of chronic kidney disease
INEM INSERM U1151, 160 Rue de Vaugirard - 75015 Paris, France.
nicolas.dupont@inserm.fr

 
Join our team: Two postdoctoral scientist positions in Cross-talk between regulatory lymphocytes and tissue-resident cells in tissue growth and repair
Vendredi 8 décembre 2023

Institut Necker Enfants Malades (INEM) is a biomedical research center located on the Hôpital Necker-Enfants Malades campus. It benefits from numerous state-of-the-art core facilities. The Campus has a long-standing reputation for scientific excellence (8 principal investigators (PIs) at INEM received ERC awards) and key pioneering medical contributions (e.g. transplantation, biotherapy, gene therapy). It provides a vibrant environment for basic research and translational innovation. Two new treatments coming out of INEM were FDA-approved in 2023.
The team “Immunity in Health and Disease” is dedicated to understanding how regulatory T and B lymphocytes interact with immune cells and tissue resident cells during health and disease. Previous work of the team led to the discovery that B cells could regulate immunity through the provision of IL-10 and IL-35 (Nature Immunology 2002, Immunity 2010, Nature 2014, Immunity 2018), and the finding that B cells contributed to the pathogenesis of autoimmune disease through the production of IL-6 (J Exp Med 2012).

The team is now interested in the interaction between regulatory T or B lymphocytes and tissue resident cells, with a focus on the central nervous system in the context of neuroinflammation and the prostate in the context of non-inflammatory pathological tissue growth. This research is based on strong background such as the establishment of a protocol to produce pure regulatory B cells that cure recipient mice from ongoing inflammatory disease in adoptive cell therapy (Science, in revision; 3 patents).

In this context, the team is seeking 2 talented post-doctoral scientists to develop the two funded projects on the central nervous system and on the prostate, respectively. The projects will involve state-of-the-art single cell transcriptomics, machine learning, multiplex imaging, and multidimensional flow cytometry to tackle the associated questions in experimental models and clinical samples. The goals of these projects are to decipher how regulatory T and B lymphocytes promote tissue repair and modulate tissue growth in inflammatory (neuroinflammation) and noninflammatory (prostate hypergrowth) diseases, with the long-term perspective of elaborating novel therapeutic strategies for such diseases.

The positions are open. Salary will be according to experience following INSERM regulation.

How to apply
Please send your application via email in English with CV, a brief outline of previous research, and contact details of three references to:
Simon Fillatreau: simon.fillatreau@inserm.fr ; 
simonfillatreau@gmail.com

INEM- Team – Immunity in Health and Disease, 156-160, rue de Vaugirard, 75015 Paris, France

Join our team: Postdoctoral position in Cell Biology of organelle biogenesis in stress response - Morel Lab
Mardi 5 décembre 2023

About the host laboratory
The host team - Cellular & Membrane Dynamics in Stress Response, Etienne Morel - is part of the Cell Biology Department at Institut Necker Enfants Malades (INEM). Our lab research is focused on molecular and cellular aspects of stress response and autophagy machinery mobilization, including membrane remodeling and trafficking.
INEM is an international biomedical research center located on the Necker campus, and supported by the INSERM, CNRS, and Université Paris Cité. The Necker campus encompasses as well the Necker-Enfants Malades Hospital and the Imagine Institute. Moreover, the Necker campus and INEM benefit from several core facilities including animal facility, histology, flow cytometry, imaging, image analysis, genomics, bioinformatics, proteomics, viral production, and metabolomics.

About the project
Autophagy is a cellular process responding to homeostasis alteration and initiated by the de novo formation of autophagosomes. Autophagosome biogenesis requires a complex sequence of events that allow for the nucleation of the phagophore from the omegasome, a transient and specialized domain of the endoplasmic reticulum (ER) membrane. These early steps of autophagy are associated with phase transition, phosphoinositides metabolism, membrane contact-sites and local lipid-delivery. Our previous work shows that ER-plasma membrane contact-sites (Nascimbeni et al. EMBO J. 2017) and ER-endosomes interface (Da Graca et al. Life Sci. Alliance 2022) are mobilized for autophagosome biogenesis in response to nutritional stress. This strongly suggests that ER spatial organization is directly connected with pre-autophagic machinery functional activation. While cytoskeletons are known in membrane trafficking, their specific involvement, especially F-actin and intermediate filaments (IFs), in phagophore formation remains unclear. Leveraging our preliminary data and literature insights, our project aims to decipher the cytoskeletons interplay at ER membrane platforms during the early stages of autophagosome biogenesis.

Your profile
We are seeking a highly motivated and creative candidate (m/f) with a strong drive for independent research.

International candidates with a PhD in molecular and cellular biology obtained during the past four years and with a strong background in the fields of membrane trafficking and autophagy are encouraged to apply.

The two-year position is available starting summer 2024.

How to apply
Please send a concise cover letter with a statement of research interest and summary of previous research activity, a detailed curriculum vitae, and two reference letters to Etienne Morel: etienne.morel@inserm.fr Applications will be considered upon submission with a deadline on March 1st, 2024.

Additional information
Key-words: autophagosome biogenesis, membrane remodeling, cytoskeletons, ER
Recent publications from the Morel Lab at the following link: https://pubmed.ncbi.nlm.nih.gov/?term=Morel+E&sort=date

Join our Team: Postdoctoral Research position in Airway inflammation in Cystic Fibrosis and CFTR correction
Mercredi 27 septembre 2023

The "Cystic fibrosis and other epithelial respiratory protein misfolding diseases" lab is excited to announce an opportunity for a dedicated and motivated postdoctoral fellow to join our research team, located at INEM - Institut Necker Enfants Malades in Paris. 
Our research aims to understand the inflammation pathways remodeled by highly efficient CFTR modulation including the protease anti-protease balance, oxidative stress and interaction with innate immune cells. This will be based on a multi-omic approach of patient primary airway cultures under different experimental conditions and study of sputum samples collected longitudinally in a pediatric cohort.

The ideal candidate will have expertise in standard molecular biology techniques and mammalian cell culture; experience in inflammation is desirable but not essential.

Highly motivated candidates with a PhD or MD, PhD are welcome to apply. Applicants should possess strong laboratory, analytical, interpersonal and communication skills.

INEM is located in the Necker Hospital campus which provides an exceptional scientific and academic environment. Administered jointly by INSERM, CNRS and the Université de Paris, INEM benefits from close contacts with clinicians and state-of-the-art technological platforms in one of France’s foremost research centered universities.

The successful candidate will be offered a 24-to-36-month contract with salary depending on experience. This position is available immediately, though start date is flexible.

Interested candidates should email a brief cover letter indicating their research interests, experience and career goals, CV, and contact information for reference to isabelle.sermet@aphp.fr

Join our Team: Postdoctoral Research position in Gene Editing in Cystic Fibrosis
Mercredi 27 septembre 2023
The "Cystic fibrosis and other epithelial respiratory protein misfolding diseases" lab is excited to announce an opportunity for a dedicated and motivated postdoctoral fellow, trained in gene editing, to join our research team, located at INEM - Institut Necker Enfants Malades in Paris.
Our research aims to restore CFTR activity in primary cells of patients with Cystic Fibrosis (CF) based on various approaches. Nonsense and splice site mutations represent a potential theratype which can be rescued by CRISPR gene editing. The aim of this project is to optimize and vectorize the Cas9/gRNAs validated in our laboratory, and confirm their ability to functionally restore CFTR function in primary cells from patients with these mutations in readiness for pre-clinical evaluation.
The ideal candidate will have expertise in standard molecular biology techniques and mammalian cell culture; experience of CRISPR Cas9 editing and/or lentiviral vectors is desirable but not essential.
Highly motivated candidates with a PhD or MD, PhD in Gene editing/CRISPR editing are welcome to apply. Applicants should possess strong laboratory, analytical, interpersonal and communication skills.
INEM is located in the Necker Hospital campus which provides an exceptional scientific and academic environment. Administered jointly by INSERM, CNRS and the Université de Paris, INEM benefits from close contacts with clinicians and state-of-the-art technological platforms in one of France’s foremost research centered universities.
The successful candidate will be offered a 24-to-36-month contract with salary depending on experience. This position is available immediately, though start date is flexible.
Interested candidates should email a brief cover letter indicating their research interests, experience and career goals, CV, and contact information for reference to alexandre.hinzpeter@inserm.fr and isabelle.sermet@aphp.fr.
Grants Project Officer at Institut Necker Enfants Malades, Université Paris Cité – INSERM - CNRS
Mardi 11 juillet 2023
Grants Project Officer at Institut Necker Enfants Malades, Université Paris Cité – INSERM - CNRS
 
The Institut Necker Enfants Malades (INEM https://www.institut-necker-enfantsmalades.fr/) is seeking a Grants Project Officer to join us on a Fixed Term contract starting in September 2023.
 
The INEM is a world-class biomedical research Institute affiliated with Université Paris Cité, CNRS and INSERM. The institute has an international staff of around 300 people from more than 20 countries, partitioned in 21 research teams and administrative support services. The INEM is located in Paris, the French capital and one of the most vibrant cultural scenes in the world. INEM teams collaboratively address multiple facets of fundamental science and translational approaches, promoting a holistic understanding of human pathophysiology that associates expertise in multiple organs and in inter-organ hormonal communications, growth, metabolism, inflammation, immunity and haemato-oncology. To achieve this, the close ties with the clinical services of the Hôpital Necker-Enfants Malades on the joint Necker campus are both a fundamental element and critical asset.
 
We need the candidate to play a key role in dealing directly with both the principal investigators and the administrative staff from the institution and be expected to identify research funding opportunities then to provide scientific advice, guidance, and recommendations to ensure successful applications and partnerships.
 
Who we are looking for:
 
  • Experience of grants management and processes.
  • A good working knowledge of project management.
  • Team player.
  • Highly organized with the ability to work under pressure to set deadlines.
Applicants should submit a single pdf file including (1) a 2-page Curriculum vitae, (2) a motivation letter (1 page max) and contact information for two referees. Applications should be submitted before July 21st 2023 to sabine.barbut@inserm.fr
Post-Doctoral Fellowship in Oncogenic Deregulation of Lymphoid Lineage Specification
Mardi 4 juillet 2023
Post-Doctoral Fellowship in Oncogenic Deregulation of Lymphoid Lineage Specification The Normal and Pathological Lymphoid Differentiation Laboratory from the Institut Necker Enfants Malades (INSERM UMR 1151 research center) is seeking for a PhD trained in hematopoietic stem cell biology
and/or translational leukemia research. Our laboratory investigates the interface between normal and leukemic lymphoid development. We combine a dual expertise in oncogenesis and developmental immunology to dissect the transcriptional and epigenetic events underlying oncogenic arrest during the specification of hematopoietic stem cells (HSC) toward the lymphoid lineage. Our research combines in vivo (HSC xenografts; PDX) and in vitro cellular (multi-lineage diff. assays) and molecular (single cell RNA sequencing, methylome profiling, genome-wide HiC, CUT&Tag) approaches through which understanding the malignant deregulation of early hemato-lymphopoiesis can lead both to targeted and personalized therapeutics. The specific aims of
the current research projects are: (i) to decipher the hierarchy of transcription factors and epigenetic regulators controlling the specification of human HSCs toward the lymphoid lineage; (ii) to characterize the 3D genome alterations caused by oncogenes involved in the pathogenesis of immature T-cell acute
lymphoblastic leukemia. Highly motivated candidates with a Ph.D. or M.D./Ph.D. in developmental immunology and/or hematology are welcome to apply. Applicants should possess strong laboratory, analytical, interpersonal and communication skills. Candidates with previous experience in stem cell biology, multi-omics and statistics are highly desirable.
The “Institut Necker Enfants Malades” (INEM) located in the Necker Hospital (Paris) provides an exceptional scientific and academic environment. Administered jointly by INSERM, CNRS and the Université de Paris, INEM benefits from close contacts with clinicians and state-of-the-art technological platforms in one of France’s foremost research centered universities.
The successful candidate will be offered a 24-to-36-month contract with salary depending on experience.
This position is available immediately, though start date is flexible.
Interested candidates should email a brief cover letter indicating their research interests, experience and career goals, CV, and contact information for 2 references to Pr Bruno Canque (bruno.canque@ephe.psl.eu).
Bruno CANQUE, M.D., Ph.D. and Vahid ASNAFI, M.D., Ph.D.
References :
. Alhaj Hussen, K., et al. (2017). Molecular and Functional Characterization of Lymphoid Progenitor Subsets Reveals a Bipartite Architecture of Human Lymphopoiesis. Immunity 47, 680-696 e688. 10.1016/j.immuni.2017.09.009.
. Cieslak, A., et al. (2020). Blueprint of human thymopoiesis reveals molecular mechanisms of stage-specific TCR enhancer activation. J Exp Med 217. 10.1084/jem.20192360.
. Andrieu, G.P, et al. (2021). PRC2 loss of function confers a targetable vulnerability to BET proteins in T-ALL.  Blood 138, 1855-1869. 10.1182/blood.2020010081.
. Touzart, A., et al. (2021). Epigenetic analysis of patients with T-ALL identifies poor outcomes and a hypomethylating agent-responsive subgroup. Science translational medicine 13. 10.1126/scitranslmed.abc4834
. Keita, S., et al. (2023). Distinct subsets of multi-lymphoid progenitors support ontogeny-related changes in human lymphopoiesis. Cell Rep 42, 112618. 10.1016/j.celrep.2023.112618
Group leader positions at Institut Necker Enfants Malades, Université Paris Cité, Paris-France
Jeudi 11 mai 2023
The Institut Necker Enfants Malades (INEM, https://www.institut-necker-enfants-malades.fr/) invites applications for two group leader positions. Excellent future prospects and an upwards-scientific track record will be the principal selection criteria. We promote equal opportunities, strongly encouraging applications from female candidates and welcome applications from early career scientists as well as established PIs who wish to join INEM.
Candidates should have an innovative research program complementing the existing strengths in one of the main areas in relation to INEM departments:
1) control of tissue growth, metabolism, organelle homeostasis and interorgan communication at the molecular, cell biology or patho-physiology level. In this context, bringing an expertise in primary cell organoids or organ-on-chip technology might be considered an asset;
2) fundamental and translational studies in immunology, microbiology with focus on host-pathogen interactions, and haematology. In this context, integrative interdisciplinary topics (e.g. interaction between immune cells or microbes and tissues, neuroimmunology, etc.) or the use of innovative methodological approaches (e.g. multiplexed spatial imaging, microfluidics, etc.) could be of particular interest.
The INEM is a world-class biomedical research Institute affiliated with Université Paris Cité, CNRS and INSERM. The institute has an international staff of around 300 people from more than 20 countries, partitioned in 21 research teams and administrative support services. The INEM is located in Paris, the French capital and one of the most vibrant cultural scenes in the world. INEM teams collaboratively address multiple facets of fundamental science and translational approaches, promoting a holistic understanding of human pathophysiology that associates expertise in multiple organs and in inter-organ hormonal communications, growth, metabolism, inflammation, immunity and haemato-oncology. To achieve this, the close ties with the clinical services of the Hôpital Necker-Enfants Malades on the joint Necker campus are both a fundamental element and critical asset.
The positions include more than 100m2 lab space with cell culture room and offices, complemented with a competitive start-up package and access to on-site state-of-the-art core facilities (https://www.sfr-necker.fr/). Assistance will be provided for obtaining a tenured position and attracting competitive European funding as well as establishing collaborations within the Necker campus, Université Paris Cité
and the broader Paris area, one of the major science hubs in Europe.
Candidates must have a PhD or MD/PhD. Applicants should submit a single pdf file including (1) a 2-page Curriculum vitae with a list of selected publications, (2) a summary of research accomplishments and future projects (4-page max), (3) a motivation statement for joining INEM (1 page max) and (4) contact information for three referees. Applications should be submitted before August 31st 2023 to directorate-inem@inserm.fr.
Shortlisted applicants will be informed by October 1st and invited on site in Autumn, followed by a second round of interviews to the international scientific advisory board. Final selection is expected to take place in December 2023. Installation will occur starting from January 2025
Adresse

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Parrain(s)
S.A.R. la Princesse Caroline de Hanovre qui, à travers la Fondation Princesse Grace, soutient déjà la recherche medicale et tout ce qui contribue à soulager les enfants malades en France et dans le monde, a accepté de s'engager à nos cotés pour que ce centre de medecine moleculaire, tourné entre autres vers les pathologies des enfants, prenne de vitesse les maladies et continue à relever les defis actuels.

INEM - Organigramme