A post-doctoral position is currently available for 14 months (renewable) in the team Chanelopathies: cystic fibrosis and other diseases, of Research Center "Institut Necker Enfants Malades
", INSERM U1151, Faculté Necker, Paris, France.
56.000 € / year
Our research concerns the mechanisms involved in Cystic Fibrosis (CF), a lethal genetic disease due to mutations in CF gene. The most frequent mutation, F508del, leads to CFTR misfolding and preferential degradation at the ER, which constitutes the primary cause of the channel functional expression defect at cell surface. Exosomes (EXs), as part of extracellular vesicle populations, have been shown to contain CFTR. Investigating the contribution of selective autophagocytosis of mutated CFTR proteins may bring further insights into in the molecular and cellular pathogenesis of CF, as well as the role of exosome in clearance of misfolded proteins from ER. The study may be extended to other PMD.
The post-doctoral project lies at the interface of biochemistry, molecular cell biology and proteomics, aiming to investigate involvement of EXs in the trafficking and function of CFTR variants. EXs biogenesis and composition, as well as CFTR metabolism will be established in non-polarized cells and epithelia in the presence and absence of CFTR expression. The role of selective autophagocytosis will be probed in parallel by genetic means (siRNA, Crispr). The project will require experience in protein biochemistry, molecular biology, various microscopic and immunochemical techniques. Collaboration with mass spectrometry team is in place.
Candidates for this project should have a solid background in cell culture, cellular, molecular biology (transfection, real time PCR, plasmid construction), and biochemistry (Western Blot, co-immunoprecipitation, immunohistochemistry, microscopy, immunoisolation of organelles, protein biochemistry). Experience in electrophysiology would be an advantage.
Interested candidates should e-mail a letter of application, including a CV and the names and addresses of at least two referees to:
- Aleksander Edelman (firstname.lastname@example.org), INSERM U1151, Paris, France - tel: +33 (0)6 24 63 34 93
- Isabelle Sermet-Gaudelus (email@example.com), INSERM U1151, Paris, France
- Gergely Lukacs (firstname.lastname@example.org), INSERM U1151, Paris, France and McGill University, Montreal Canada
Cystic fibrosis, protein misfolded diseases, exosome, epithelium, lung, CFTR, quality control, inflammation, infection, protein, biochemistry, microRNA, proteomics