Institut Necker Enfants Malades

Postdoctoral position in Immunology - Mahévas Lab

Sunday 26 of November, 2023

Scientific goal
Inflammatory bowel diseases (IBD), including Crohn’s disease (CD) and Ulcerative Colitis, are immune-related disorders characterized by inappropriate gut inflammation. Their incidence has increased dramatically in Western countries in recent decades. Despite the development of new treatments, IBD are insufficiently controlled in a large proportion of patients, inducing an alteration in the quality of life and a socioeconomic burden. A better understanding of the pathogenesis and identification of new therapeutic targets are therefore urgently needed.
Pathophysiology of CD is complex and not fully understood. While CD can affect the entire gastrointestinal tract, it primarily affects the terminal ileum. The underlying mechanistic reasons of this preferential location is unknown.
Patients with isolated ileal CD have higher prevalence of certain circulating IgG toward elements of the microbiota pointing toward a dysregulated humoral immune response. These antibodies are associated with the ileal location of CD as well as with a complicated phenotype (intestinal stenosis and fistulae). Cellular determinant of these humoral response in ileal CD are yet to be unraveled. Techniques to study B cells at the single cell levels have tremendously evolved these recent years and our laboratory has developed an expertise in most of these techniques.
The objectives of our study are to comprehensively characterize the humoral B cell response specifically in patients with isolated and/or complicated ileal CD.
Partners involved
Institut Necker Enfants Malades
INSERM
Université Paris Cité
Assistance Publique - Hôpitaux de Paris
Institut Mondor de Recherche Biomédicale
The post-doctoral scientist will work in the Mahevas’s Lab located at Institut Necker Enfants Malades (INEM) Research Center in the heart of Paris and at Institut Mondor de Recherche Biomédicale, in close collaboration with the clinical site (Hôpital Henri Mondor, Créteil).
Strategy
The study will be primarily performed from human sample, collected from ileal, colonic biopsies, and blood. Advanced B-cell focused single-cell techniques (including BCRseq, single-cell seq, FACS and B cell culture) mastered in our lab will be used.
Role Description
This is a full-time on-site role for a Postdoctoral Researcher in Immunology located at the INEM - Institut Necker Enfants Malades In Paris (Team Mahevas) and at Institut Mondor de Recherche Biomédicale (Team France Pirenne), in close collaboration with the clinical site (Hôpital Henri Mondor).
The Postdoctoral Researcher will be responsible for conducting independent research in immunology and applying advanced techniques to analyze complex data. They will be expected to collaborate with other researchers, PI of the project (Mathieu Uzzan) head of the lab (Matthieu Mahevas), senior scientists (Pascal Chappert, Jean Claude Weil), other postdocs, PhD students, engineers and technicians. They will also interact with other labs in France and abroad and with several platforms.
Selected candidate will attend seminars and meetings, and present their findings. Additionally, they will be responsible for writing scientific papers and grant proposals.
The position is vacant from March 2024 on a full-time basis and is integrated in an ambitious ANR-funded project (PI: Mathieu Uzzan)
Qualifications

Recent Ph.D. in Immunology or a related field
Strong knowledge in immunology
Experience in molecular biology, biochemistry, and cellular immunology techniques
Demonstrated expertise in designing and executing experiments, including flow cytometry and microscopy
Experience in data analysis
Excellent written and oral communication skills
Ability to work independently and collaboratively in a team environment
Strong organizational and time-management skills
Experience with manuscript and grant writing

Qualified candidates should e-mail before December 30th 2023 a cover letter, curriculum vitae, and list of two references to Dr Mathieu Uzzan at mathieuuzzan@gmail.com.

Join our team: Postdoctoral position in urology - Goffin Lab

Thursday 2^nd of November, 2023

About the host laboratory

The Goffin Lab (PRL/GH Pathophysiology - Translational Approaches) has >25-year international visibility in onco-endocrinology and develops research projects related to the resistance to treatment of hormone-dependent tumors (breast, prostate). The group currently involves 16 people including tenure basic scientists, clinicians and technicians, post-docs, graduate & undergraduate students.

About the project

Fully-funded project supported by the French National Agency of Research (ANR), built on solid data involving luminal progenitor-like LSC^med cells discovered by our group in a mouse model of benign prostatic hyperplasia (BPH). LSC^med cells combine androgen-independence, stem/progenitor properties, cell plasticity and therapeutic resistance (Baurès et al, Nature Reviews Urology 2022). The candidate will use various preclinical models of BPH including Pb-PRL mice, mouse- and patient-derived organoids, cell lines, and human tissue biocollections to elucidate LSC^med cell dialog with microenvironment and challenge innovative targeting strategies. The project involves established collaborative networks of biologists, immunologists, urologists, pathologists and bioinformaticians.

Your profile

We are seeking a highly motivated and creative candidate (m/f) with a strong drive for independent research. The ideal candidate will have expertise in animal, cell, and molecular biology, encompassing techniques such as histology, IHC/IF, cell sorting, cell culture, organoids, qPCR, immunoblots, and more. All applicants are expected to benefit from excellent written and oral communication skills. Proficiency in bioinformatics and/or spatial biology would be a valuable addition.
The selected candidate will be offered a one-year contract renewable up to 2.5 years.

How to apply

Please submit your application including a concise statement of research interest and CV as soon as possible but not later than December 15^th, 2023 for a start date Q1 of 2024. Applications will be considered upon submission.

Application contact: Vincent Goffin (Team leader): vincent.goffin@inserm.fr

Curious or have questions? Don't hesitate to reach out to us for an informal chat.

Additional information
Keywords: Benign prostate hyperplasia, immune-epithelial dialog, stem/progenitor properties, therapeutic targeting, scRNA-seq, spatial biology, organoids, mouse/human models

Join our team: Postdoctoral position in oncology - Goffin Lab

Thursday 2^nd of November, 2023

About the host laboratory

The Goffin Lab has >25-year international visibility in onco-endocrinology and develops research projects related to the resistance to treatment of hormone-dependent tumors (breast, prostate). The group currently involves 16 people including tenure basic scientists, clinicians and technicians, post-docs, graduate & undergraduate students.

About the project

Fully-funded project supported by the French National Institute of Cancer (INCa), built on solid data involving luminal progenitor-like LSCmed cells (first isolated by our group) that combine castration-tolerance, stem/progenitor properties, cell plasticity and tumor-initiating capacities (Baurès et al, Nature Reviews Urology 2022). The candidate will use preclinical models of prostate cancer/CRPC including transgenic mice, mouse- and patient-tumor-derived organoids, cell lines, and patient biocollections to elucidate LSCmed cell plasticity underlying treatment escape, and challenge innovative targeting strategies. This project involves established collaborative networks of biologists, urologists, pathologists and bioinformaticians.

Your profile

How to apply

Please submit your application including a concise statement of research interest and CV as soon as possible but not later than December 15^th, 2023, for a start date Q1 of 2024. Applications will be considered upon submission.

Application contact: Vincent Goffin (Team leader): vincent.goffin@inserm.fr

Curious or have questions? Don't hesitate to reach out to us for an informal chat.

Additional information
Keywords: Prostate cancer, castration-resistance, stem/progenitor cells, cell plasticity, therapeutic targeting, scRNA-seq, spatial biology, organoids, mouse/human models.

Join our Team: Postdoctoral Research position in Airway inflammation in Cystic Fibrosis and CFTR correction

Wednesday 27 of September, 2023

The "Cystic fibrosis and other epithelial respiratory protein misfolding diseases" lab is excited to announce an opportunity for a dedicated and motivated postdoctoral fellow to join our research team, located at INEM - Institut Necker Enfants Malades in Paris.
Our research aims to understand the inflammation pathways remodeled by highly efficient CFTR modulation including the protease anti-protease balance, oxidative stress and interaction with innate immune cells. This will be based on a multi-omic approach of patient primary airway cultures under different experimental conditions and study of sputum samples collected longitudinally in a pediatric cohort.

The ideal candidate will have expertise in standard molecular biology techniques and mammalian cell culture; experience in inflammation is desirable but not essential.

Highly motivated candidates with a PhD or MD, PhD are welcome to apply. Applicants should possess strong laboratory, analytical, interpersonal and communication skills.

INEM is located in the Necker Hospital campus which provides an exceptional scientific and academic environment. Administered jointly by INSERM, CNRS and the Université de Paris, INEM benefits from close contacts with clinicians and state-of-the-art technological platforms in one of France’s foremost research centered universities.

The successful candidate will be offered a 24-to-36-month contract with salary depending on experience. This position is available immediately, though start date is flexible.

Interested candidates should email a brief cover letter indicating their research interests, experience and career goals, CV, and contact information for reference to isabelle.sermet@aphp.fr

Join our Team: Postdoctoral Research position in Gene Editing in Cystic Fibrosis

Wednesday 27 of September, 2023

The "Cystic fibrosis and other epithelial respiratory protein misfolding diseases" lab is excited to announce an opportunity for a dedicated and motivated postdoctoral fellow, trained in gene editing, to join our research team, located at INEM - Institut Necker Enfants Malades in Paris.
Our research aims to restore CFTR activity in primary cells of patients with Cystic Fibrosis (CF) based on various approaches. Nonsense and splice site mutations represent a potential theratype which can be rescued by CRISPR gene editing. The aim of this project is to optimize and vectorize the Cas9/gRNAs validated in our laboratory, and confirm their ability to functionally restore CFTR function in primary cells from patients with these mutations in readiness for pre-clinical evaluation.
The ideal candidate will have expertise in standard molecular biology techniques and mammalian cell culture; experience of CRISPR Cas9 editing and/or lentiviral vectors is desirable but not essential.
Highly motivated candidates with a PhD or MD, PhD in Gene editing/CRISPR editing are welcome to apply. Applicants should possess strong laboratory, analytical, interpersonal and communication skills.
INEM is located in the Necker Hospital campus which provides an exceptional scientific and academic environment. Administered jointly by INSERM, CNRS and the Université de Paris, INEM benefits from close contacts with clinicians and state-of-the-art technological platforms in one of France’s foremost research centered universities.
The successful candidate will be offered a 24-to-36-month contract with salary depending on experience. This position is available immediately, though start date is flexible.
Interested candidates should email a brief cover letter indicating their research interests, experience and career goals, CV, and contact information for reference to alexandre.hinzpeter@inserm.fr and isabelle.sermet@aphp.fr.

Grants Project Officer at Institut Necker Enfants Malades, Université Paris Cité – INSERM - CNRS

Tuesday 11 of July, 2023

Grants Project Officer at Institut Necker Enfants Malades, Université Paris Cité – INSERM - CNRS

The Institut Necker Enfants Malades (INEM https://www.institut-necker-enfantsmalades.fr/) is seeking a Grants Project Officer to join us on a Fixed Term contract starting in September 2023.

The INEM is a world-class biomedical research Institute affiliated with Université Paris Cité, CNRS and INSERM. The institute has an international staff of around 300 people from more than 20 countries, partitioned in 21 research teams and administrative support services. The INEM is located in Paris, the French capital and one of the most vibrant cultural scenes in the world. INEM teams collaboratively address multiple facets of fundamental science and translational approaches, promoting a holistic understanding of human pathophysiology that associates expertise in multiple organs and in inter-organ hormonal communications, growth, metabolism, inflammation, immunity and haemato-oncology. To achieve this, the close ties with the clinical services of the Hôpital Necker-Enfants Malades on the joint Necker campus are both a fundamental element and critical asset.

We need the candidate to play a key role in dealing directly with both the principal investigators and the administrative staff from the institution and be expected to identify research funding opportunities then to provide scientific advice, guidance, and recommendations to ensure successful applications and partnerships.

Who we are looking for:

Experience of grants management and processes.
A good working knowledge of project management.
Team player.
Highly organized with the ability to work under pressure to set deadlines.

Applicants should submit a single pdf file including (1) a 2-page Curriculum vitae, (2) a motivation letter (1 page max) and contact information for two referees. Applications should be submitted before July 21st 2023 to sabine.barbut@inserm.fr

Post-Doctoral Fellowship in Oncogenic Deregulation of Lymphoid Lineage Specification

Tuesday 4 of July, 2023

Post-Doctoral Fellowship in Oncogenic Deregulation of Lymphoid Lineage Specification The Normal and Pathological Lymphoid Differentiation Laboratory from the Institut Necker Enfants Malades (INSERM UMR 1151 research center) is seeking for a PhD trained in hematopoietic stem cell biology
and/or translational leukemia research. Our laboratory investigates the interface between normal and leukemic lymphoid development. We combine a dual expertise in oncogenesis and developmental immunology to dissect the transcriptional and epigenetic events underlying oncogenic arrest during the specification of hematopoietic stem cells (HSC) toward the lymphoid lineage. Our research combines in vivo (HSC xenografts; PDX) and in vitro cellular (multi-lineage diff. assays) and molecular (single cell RNA sequencing, methylome profiling, genome-wide HiC, CUT&Tag) approaches through which understanding the malignant deregulation of early hemato-lymphopoiesis can lead both to targeted and personalized therapeutics. The specific aims of
the current research projects are: (i) to decipher the hierarchy of transcription factors and epigenetic regulators controlling the specification of human HSCs toward the lymphoid lineage; (ii) to characterize the 3D genome alterations caused by oncogenes involved in the pathogenesis of immature T-cell acute
lymphoblastic leukemia. Highly motivated candidates with a Ph.D. or M.D./Ph.D. in developmental immunology and/or hematology are welcome to apply. Applicants should possess strong laboratory, analytical, interpersonal and communication skills. Candidates with previous experience in stem cell biology, multi-omics and statistics are highly desirable.
The “Institut Necker Enfants Malades” (INEM) located in the Necker Hospital (Paris) provides an exceptional scientific and academic environment. Administered jointly by INSERM, CNRS and the Université de Paris, INEM benefits from close contacts with clinicians and state-of-the-art technological platforms in one of France’s foremost research centered universities.
The successful candidate will be offered a 24-to-36-month contract with salary depending on experience.
This position is available immediately, though start date is flexible.
Interested candidates should email a brief cover letter indicating their research interests, experience and career goals, CV, and contact information for 2 references to Pr Bruno Canque (bruno.canque@ephe.psl.eu).
Bruno CANQUE, M.D., Ph.D. and Vahid ASNAFI, M.D., Ph.D.
References :
. Alhaj Hussen, K., et al. (2017). Molecular and Functional Characterization of Lymphoid Progenitor Subsets Reveals a Bipartite Architecture of Human Lymphopoiesis. Immunity 47, 680-696 e688. 10.1016/j.immuni.2017.09.009.
. Cieslak, A., et al. (2020). Blueprint of human thymopoiesis reveals molecular mechanisms of stage-specific TCR enhancer activation. J Exp Med 217. 10.1084/jem.20192360.
. Andrieu, G.P, et al. (2021). PRC2 loss of function confers a targetable vulnerability to BET proteins in T-ALL. Blood 138, 1855-1869. 10.1182/blood.2020010081.
. Touzart, A., et al. (2021). Epigenetic analysis of patients with T-ALL identifies poor outcomes and a hypomethylating agent-responsive subgroup. Science translational medicine 13. 10.1126/scitranslmed.abc4834
. Keita, S., et al. (2023). Distinct subsets of multi-lymphoid progenitors support ontogeny-related changes in human lymphopoiesis. Cell Rep 42, 112618. 10.1016/j.celrep.2023.112618

Group leader positions at Institut Necker Enfants Malades, Université Paris Cité, Paris-France

Thursday 11 of May, 2023

The Institut Necker Enfants Malades (INEM, https://www.institut-necker-enfants-malades.fr/) invites applications for two group leader positions. Excellent future prospects and an upwards-scientific track record will be the principal selection criteria. We promote equal opportunities, strongly encouraging applications from female candidates and welcome applications from early career scientists as well as established PIs who wish to join INEM.
Candidates should have an innovative research program complementing the existing strengths in one of the main areas in relation to INEM departments:
1) control of tissue growth, metabolism, organelle homeostasis and interorgan communication at the molecular, cell biology or patho-physiology level. In this context, bringing an expertise in primary cell organoids or organ-on-chip technology might be considered an asset;
2) fundamental and translational studies in immunology, microbiology with focus on host-pathogen interactions, and haematology. In this context, integrative interdisciplinary topics (e.g. interaction between immune cells or microbes and tissues, neuroimmunology, etc.) or the use of innovative methodological approaches (e.g. multiplexed spatial imaging, microfluidics, etc.) could be of particular interest.
The INEM is a world-class biomedical research Institute affiliated with Université Paris Cité, CNRS and INSERM. The institute has an international staff of around 300 people from more than 20 countries, partitioned in 21 research teams and administrative support services. The INEM is located in Paris, the French capital and one of the most vibrant cultural scenes in the world. INEM teams collaboratively address multiple facets of fundamental science and translational approaches, promoting a holistic understanding of human pathophysiology that associates expertise in multiple organs and in inter-organ hormonal communications, growth, metabolism, inflammation, immunity and haemato-oncology. To achieve this, the close ties with the clinical services of the Hôpital Necker-Enfants Malades on the joint Necker campus are both a fundamental element and critical asset.
The positions include more than 100m2 lab space with cell culture room and offices, complemented with a competitive start-up package and access to on-site state-of-the-art core facilities (https://www.sfr-necker.fr/). Assistance will be provided for obtaining a tenured position and attracting competitive European funding as well as establishing collaborations within the Necker campus, Université Paris Cité
and the broader Paris area, one of the major science hubs in Europe.
Candidates must have a PhD or MD/PhD. Applicants should submit a single pdf file including (1) a 2-page Curriculum vitae with a list of selected publications, (2) a summary of research accomplishments and future projects (4-page max), (3) a motivation statement for joining INEM (1 page max) and (4) contact information for three referees. Applications should be submitted before August 31st 2023 to directorate-inem@inserm.fr.
Shortlisted applicants will be informed by October 1st and invited on site in Autumn, followed by a second round of interviews to the international scientific advisory board. Final selection is expected to take place in December 2023. Installation will occur starting from January 2025

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